|
KMID : 1007520110200010243
|
|
Food Science and Biotechnology
2011 Volume.20 No. 1 p.243 ~ p.249
|
|
|
Purification and Characterization of a 6.5 kDa Antioxidant Peptidoglycan Purified from Silk Worm (Bombyx mori) Pupae Extract
|
|
Rehab Ahmed Al-Azzouny
Wang Ren
Yoo Sang-Ho
|
|
|
Abstract
|
|
|
|
A unique antioxidant peptidoglycan of 6.5 kDa was purified from silk worm (Bombyx mori) pupae. The peptidoglycan was extracted and isolated from the acidified aqueous extract of the homogenized pupae by chloroform/ methanol (2:1, v/v), and purified by size-exclusion chromatography and reverse-phase high-performance liquid chromatography (RP-HPLC). This separation method allowed an easy and consistent preparation of the peptidoglycan from the high protein load by using only 3 steps of purification. The highly pure peptidoglycan consisted of fucose, glucosamine, galactosamine, glucose, and galactose as constituent sugars when revealed by high-performance anion exchange chromatography (HPAEC) analysis. The amino acid composition analysis showed the presence of acidic and polar amino acids (51.7%), hydrophobic (37.2%), and basic amino acids (9.3%) in the purified peptidoglycan. The IC50 of the purified peptidoglycan was 0.22±0.05 and 0.61±0.09 μg for diphenyl-2-picryl hydrazyl (DPPH) and 2,2'-azinobis-(3-ethylbenzothiazoline- 6-sulfonic acid) (ABTS), respectively, as quantified by Ophthalaldehyde (OPA) microplate fluorescent assay.
|
|
|
KEYWORD
|
|
|
Bombyx mori pupae, peptidoglycan, 2, 2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), diphenyl-2-picryl hydrazyl (DPPH), antioxidant
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|